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Proceedings Paper

Selective labeling of a single organelle by using two-photon conversion of a photoconvertible fluorescent protein
Author(s): Wataru Watanabe; Tomoko Shimada; Sachihiro Matsunaga; Daisuke Kurihara; Shin-ichi Arimura; Nobuhiro Tsutsumi; Kiichi Fukui; Kazuyoshi Itoh
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Paper Abstract

We present space-selective labeling of organelles by using two-photon conversion of a photoconvertible fluorescent protein with near-infrared femtosecond laser pulses. Two-photon excitation of photoconvertible fluorescent-protein, Kaede, enables space-selective labeling of organelles. We alter the fluorescence of target mitochondria in a tobacco BY-2 cell from green to red by focusing femtosecond laser pulses with a wavelength of 750 nm.

Paper Details

Date Published: 15 February 2008
PDF: 6 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601B (15 February 2008); doi: 10.1117/12.768745
Show Author Affiliations
Wataru Watanabe, National Institute of Advanced Industrial Science and Technology (Japan)
Tomoko Shimada, Osaka Univ. (Japan)
Sachihiro Matsunaga, Osaka Univ. (Japan)
Daisuke Kurihara, Osaka Univ. (Japan)
Shin-ichi Arimura, Univ. of Tokyo (Japan)
Nobuhiro Tsutsumi, Univ. of Tokyo (Japan)
Kiichi Fukui, Osaka Univ. (Japan)
Kazuyoshi Itoh, Osaka Univ. (Japan)


Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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