Share Email Print

Proceedings Paper

Fluorescence enhancement on silver nanostructures: studies of components of ribosomal translation in vitro
Author(s): Wlodek Mandecki; Shashank Bharill; Julian Borejdo; Diana Cabral; Barry S. Cooperman; Ian Farrell; Linus Fetter; Emanuel Goldman; Zygmunt Gryczynski; Hieronim Jakubowski; Hanqing Liu; Rafal Luchowski; Evgenia Matveeva; Dongli Pan; Haiou Qin; Donald Tennant; Ignacy Gryczynski
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Metallic particles, silver in particular, can significantly enhance the fluorescence of dye molecules in the immediate vicinity (5-20 nm) of the particle. This magnifying effect can be theoretically explained/predicted by considering the change of photonic mode density near the fluorophore due to coupling to the conducting surface. We are using this method to observe fluorescence from a single ribosomal particle in a project aimed at acquiring sequence information from the translating ribosome (NIH's $1000 Genome Initiative). Several quartz slides with silver nanostructures were made using electron beam lithography techniques. The structures were approximately 50 nm high silver tiles measuring 400-700 nm on the side, and were spaced differently over a total area of 1 mm x 1 mm on any given quartz slide. In a preliminary experiment, we coated this surface with the Alexa 647-labeled antibodies and collected single molecule images using the MicroTime 200 (PicoQuant) confocal system. We showed that the fluorescence intensity measured over the silver islands film was more than 100-fold higher than fluorescence from a comparable site on uncoated section of the quartz slide. No noticeable photobleaching was seen. The fluorescence lifetime was very short, about 200 ps or less (this is the resolution limit of the system). The method has great promise for investigations of biologically relevant single molecules.

Paper Details

Date Published: 21 February 2008
PDF: 12 pages
Proc. SPIE 6862, Single Molecule Spectroscopy and Imaging, 68620T (21 February 2008); doi: 10.1117/12.768612
Show Author Affiliations
Wlodek Mandecki, UMDNJ, New Jersey Medical School (United States)
Shashank Bharill, Univ. of North Texas Health Science Ctr. (United States)
Julian Borejdo, Univ. of North Texas Health Science Ctr. (United States)
Diana Cabral, Univ. of Pennsylvania (United States)
Barry S. Cooperman, Univ. of Pennsylvania (United States)
Ian Farrell, Univ. of Pennsylvania (United States)
Linus Fetter, Alcatel-Lucent Technologies (United States)
Emanuel Goldman, UMDNJ, New Jersey Medical School (United States)
Zygmunt Gryczynski, Univ. of North Texas Health Science Ctr. (United States)
Hieronim Jakubowski, UMDNJ, New Jersey Medical School (United States)
Hanqing Liu, Univ. of Pennsylvania (United States)
Rafal Luchowski, Univ. of North Texas Health Science Ctr. (United States)
Evgenia Matveeva, Univ. of North Texas Health Science Ctr. (United States)
Dongli Pan, Univ. of Pennsylvania (United States)
Haiou Qin, Univ. of Pennsylvania (United States)
Donald Tennant, Alcatel-Lucent Technologies (United States)
Ignacy Gryczynski, Univ. of North Texas Health Science Ctr. (United States)

Published in SPIE Proceedings Vol. 6862:
Single Molecule Spectroscopy and Imaging
Jörg Enderlein; Zygmunt K. Gryczynski; Rainer Erdmann, Editor(s)

© SPIE. Terms of Use
Back to Top