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Proceedings Paper

Internalization of fluorescent dextrans in the submandibular salivary glands of live animals: a study combining intravital two-photon microscopy and second harmonic generation
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Paper Abstract

Here we show that a combination of two-photon microscopy and second harmonic generation can be successfully used to study endocytosis in the submandibular salivary glands of live animals. First, we have characterized the threedimensional structure of the acini and the ducts forming the parenchyma of the excised glands by exciting various endogenous molecules, which highlight the shape of the cells and various components of the extracellular matrix. Next, by time-lapse imaging we show the dynamic distribution of fluorescent probes injected systemically. This was achieved by using a custom-made holder aimed to reduce the motion artifacts associated with the heartbeat and the respiration in the live animals. Finally, we show that fluorescent dextrans are internalized primarily by the supporting cells in the salivary glands, a characteristic shared by other secretory organs such as the pancreas.

Paper Details

Date Published: 15 February 2008
PDF: 12 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601V (15 February 2008); doi: 10.1117/12.768051
Show Author Affiliations
Andrius Masedunskas, National Institutes of Health (United States)
Roberto Weigert, National Institutes of Health (United States)


Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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