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Proceedings Paper

Non-invasive monitoring and quantitative analysis of patch test reactions by reflectance spectrophotometry, laser Doppler flowmetry and transepidermal water loss
Author(s): Natalja Skrebova Eikje M.D.; Seiji Arase
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Paper Abstract

Reflectance spectrophotometry (RS), laser Doppler flowmetry (LDF) and transepidermal water loss (TEWL) techniques were simultaneously used to non-invasively monitor skin colour (SC), skin blood flow (SBF) and barrier function damage (BFD) in routinely patch-tested Japanese patients in dermatology clinic. The analytical quality, reliability and reproducibility of each technique were compared and analyzed in correlated to visual scoring patch test (PT) reactions as negative (-), doubtful (+?), weak (+) and strong (++/+++) at 48- and 72-hour monitoring. An attempt was made to quantify predominant in the clinic "+?"- and "+'"-PT-reactions. The relationship between 48 h and 72 h measurements in different reaction groups was poor for TEWL, LDF showed a tendency to decrease at 72 h, but good for RS. A correlation between visual scorings and instrumental mean values was poor for TEWL, good for LDF and excellent for RS. So, measurements by RS were the most statistically significant to non-invasively monitor and quantify doubtful, weak and strong PT reactions, accordingly providing continuous data grading of reaction intensity suitable in the clinic. Moreover, monitoring of SC changes was the most reliable parameter for the quantitative distinguishing of doubtful and weak reactions in pigmented skin.

Paper Details

Date Published: 22 February 2008
PDF: 12 pages
Proc. SPIE 6864, Biomedical Applications of Light Scattering II, 68640E (22 February 2008); doi: 10.1117/12.765816
Show Author Affiliations
Natalja Skrebova Eikje M.D., Waseda Univ. (Japan)
The Univ. of Tokushima School of Medicine (Japan)
Seiji Arase, The Univ. of Tokushima School of Medicine (Japan)

Published in SPIE Proceedings Vol. 6864:
Biomedical Applications of Light Scattering II
Adam Wax; Vadim Backman, Editor(s)

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