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Proceedings Paper

Direct electrical detection of target cells on a microfluidic biochip
Author(s): Mehdi Javanmard; AmirAli H. Talasaz; Mohsen Nemat-Gorgani; Fabian Pease; Mostafa Ronaghi; Ronald W. Davis
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Paper Abstract

Pathogenic bacterial cell detection is currently performed using techniques such as culture enrichment and various plating methods, which are expensive and can take up to several days. In this study, we describe the design, fabrication, and testing of a rapid and inexpensive sensor for detection of target cells electrically in real-time. The sensor operates with the use of microelectrodes integrated in a micro-channel. As a proof of principle, we have successfully demonstrated real-time detection of target yeast cells with a concentration of 107 cells/ml. We have also demonstrated the selectivity of our sensors in responding to target cells while remaining irresponsive to non-target cells. We also perform theoretical modeling in order to determine the ultimate detection limit of the sensor. Based on our modeling results, proper optimization of the sensor can yield detection limits approaching the single cell level.

Paper Details

Date Published: 12 February 2008
PDF: 10 pages
Proc. SPIE 6886, Microfluidics, BioMEMS, and Medical Microsystems VI, 68860B (12 February 2008); doi: 10.1117/12.765611
Show Author Affiliations
Mehdi Javanmard, Stanford Univ. (United States)
Stanford Genome Technology Ctr. (United States)
AmirAli H. Talasaz, Stanford Univ. (United States)
Stanford Genome Technology Ctr. (United States)
Mohsen Nemat-Gorgani, Stanford Genome Technology Ctr. (United States)
Fabian Pease, Stanford Univ. (United States)
Mostafa Ronaghi, Stanford Genome Technology Ctr. (United States)
Ronald W. Davis, Stanford Genome Technology Ctr. (United States)


Published in SPIE Proceedings Vol. 6886:
Microfluidics, BioMEMS, and Medical Microsystems VI
Wanjun Wang; Claude Vauchier, Editor(s)

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