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Proceedings Paper

Corneal imaging by second and third harmonic generation microscopy
Author(s): Arnaud Brocas; Louis Jay; Eric Mottay; Isabelle Brunette; Tsuneyuki Ozaki
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Paper Abstract

Advanced imaging methods are essential tools for improved outcome of refractive surgery. Second harmonic generation (SHG) and third harmonic generation (THG) microscopy are noninvasive high-resolution imaging methods, which can discriminate the different layers of the cornea, thus having strong impact on the outcome of laser surgery. In this work, we use an Ytterbium femtosecond laser as the laser source, the longer wavelength of which reduces scattering, and allows simultaneous SHG and THG imaging. We present SHG and THG images and profiles of pig corneas that clearly show the anterior surface of the cornea, the entry in the stroma and its end, and the posterior surface of the cornea. These observations allow localizing the epithelium, the stroma and the endothelium. Other experiments give information about the structure and cytology of the corneal layers.

Paper Details

Date Published: 15 February 2008
PDF: 9 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68600C (15 February 2008); doi: 10.1117/12.764432
Show Author Affiliations
Arnaud Brocas, Institut National de la Recherche Scientifique (Canada)
Louis Jay, Institut National de la Recherche Scientifique (Canada)
Eric Mottay, Amplitude Systemes (France)
Isabelle Brunette, BioFemto Vision Canada, Hopital Maisonneuve Rosemont (Canada)
Tsuneyuki Ozaki, Institut National de la Recherche Scientifique (Canada)


Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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