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Proceedings Paper

Utilizing two-photon fluorescence and second harmonic generation microscopy to study human bone marrow mesenchymal stem cell morphogenesis in chitosan scaffold
Author(s): Ping-Jung Su; Chi-Hsiu Huang; Yi-You Huang; Hsuan-Sue Lee; Chen-Yuan Dong
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Paper Abstract

A major goal of tissue engineering is to cultivate the cartilage in vitro. One approach is to implant the human bone marrow mesenchymal stem cells into the three dimensional biocompatible and biodegradable material. Through the action of the chondrogenic factor TGF-β3, the stem cells can be induced to secrete collagen. In this study, mesenchymal stem cells are implanted on the chitosan scaffold and TGF-β3 was added to produce the cartilage tissue and TP autofluorescence and SHG microscopy was used to image the process of chondrogenesis. With additional development, multiphoton microscopy can be developed into an effective tool for evaluating the quality of tissue engineering products.

Paper Details

Date Published: 6 February 2008
PDF: 5 pages
Proc. SPIE 6858, Optics in Tissue Engineering and Regenerative Medicine II, 68580H (6 February 2008); doi: 10.1117/12.764411
Show Author Affiliations
Ping-Jung Su, National Taiwan Univ. (Taiwan)
Chi-Hsiu Huang, National Taiwan Univ. (Taiwan)
Yi-You Huang, National Taiwan Univ. (Taiwan)
Hsuan-Sue Lee, National Taiwan Univ. Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6858:
Optics in Tissue Engineering and Regenerative Medicine II
Sean J. Kirkpatrick; Ruikang K. Wang, Editor(s)

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