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Proceedings Paper

Real-time image mosaicing with a hand-held dual-axes confocal microscope
Author(s): Kevin Loewke; David Camarillo; Wibool Piyawattanametha; David Breeden; Kenneth Salisbury
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Paper Abstract

In recent years there has been growing interest in using confocal microscopy to observe tissue structure and function for in vivo pathology. Although confocal microscopy can provide image resolution that is comparable to histopathology, it can be limited by a small field-of-view as well as a low signal-to-noise ratio. In this paper we show that image mosaicing can enhance confocal microscopy by stitching multiple images together to widen the field-of-view and increase the signal-to-noise ratio. Specifically, we present a real-time image mosaicing system for imaging human skin with a hand-held dual-axes confocal microscope. Our system allows the user to "paint" an image mosaic in real-time and aids navigation by localizing the current view with respect to the larger image map. We first discuss image calibration, then describe an efficient algorithm for real-time image mosaicing, and finally present experimental results obtained in vivo with a dual-axes confocal microscope.

Paper Details

Date Published: 21 February 2008
PDF: 9 pages
Proc. SPIE 6851, Endoscopic Microscopy III, 68510F (21 February 2008); doi: 10.1117/12.764322
Show Author Affiliations
Kevin Loewke, Stanford Univ. (United States)
David Camarillo, Stanford Univ. (United States)
Wibool Piyawattanametha, Stanford Univ. (United States)
David Breeden, Stanford Univ. (United States)
Kenneth Salisbury, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 6851:
Endoscopic Microscopy III
Guillermo J. Tearney; Thomas D. Wang, Editor(s)

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