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Proceedings Paper

Contrast enhancement of laser speckle skin image: use of optical clearing agent in conjunction with micro-needling
Author(s): Taeyoon Son; Jinhee Yoon; Chang-Yong Ko; Yong-Heum Lee; Kiwoon Kwon; Han Sung Kim; Kyoung Joung Lee; Byungjo Jung
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Paper Abstract

Laser speckle imaging modality is one of widely used methods to evaluate blood flow because of its simplicity. However, laser speckle image has a limitation in the evaluation of subcutaneous blood flow due to its low contrast perfusion image. Various methods have been tried to enhance the perfusion image contrast. Such methods presented positive results in some degree. However, it could not be fundamental solutions due to low penetration depth of lasers restricted by optical tissue scattering property. This study suggests a method to enhance the perfusion image contrast of laser speckle imaging modality by increasing the penetration depth of lasers. An optical clearing agent (glycerol) was topically applied on skin treated with micro-needle roller in order to reduce the time period of optical tissue clearing and therefore, enhance the penetration depth of laser. In this study, we investigated the effect of glycerol and micro-needling methods in the contrast enhancement of laser speckle perfusion skin image and presented the results of in-vitro and in-vivo animal experiment.

Paper Details

Date Published: 8 February 2008
PDF: 7 pages
Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 68420E (8 February 2008); doi: 10.1117/12.764308
Show Author Affiliations
Taeyoon Son, Yonsei Univ. (South Korea)
Jinhee Yoon, Yonsei Univ. (South Korea)
Chang-Yong Ko, Yonsei Univ. (South Korea)
Yong-Heum Lee, Yonsei Univ. (South Korea)
Kiwoon Kwon, Yonsei Univ. (South Korea)
Han Sung Kim, Yonsei Univ. (South Korea)
Kyoung Joung Lee, Yonsei Univ. (South Korea)
Byungjo Jung, Yonsei Univ. (South Korea)

Published in SPIE Proceedings Vol. 6842:
Photonic Therapeutics and Diagnostics IV
Kenton W. Gregory; Guillermo J. Tearney; Reza S. Malek; Nikiforos Kollias; Bernard Choi; Haishan Zeng; Brian Jet-Fei Wong; Justus F. R. Ilgner; Henry Hirschberg; Steen J. Madsen, Editor(s)

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