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Proceedings Paper

In vivo multiphoton imaging of bile duct ligation
Author(s): Yuan Liu; Feng-Chieh Li; Hsiao-Chin Chen; Po-shou Chang; Shu-Mei Yang; Hsuan-Shu Lee; Chen-Yuan Dong
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Paper Abstract

Bile is the exocrine secretion of liver and synthesized by hepatocytes. It is drained into duodenum for the function of digestion or drained into gallbladder for of storage. Bile duct obstruction is a blockage in the tubes that carry bile to the gallbladder and small intestine. However, Bile duct ligation results in the changes of bile acids in serum, liver, urine, and feces1, 2. In this work, we demonstrate a novel technique to image this pathological condition by using a newly developed in vivo imaging system, which includes multiphoton microscopy and intravital hepatic imaging chamber. The images we acquired demonstrate the uptake, processing of 6-CFDA in hepatocytes and excretion of CF in the bile canaliculi. In addition to imaging, we can also measure kinetics of the green fluorescence intensity.

Paper Details

Date Published: 15 February 2008
PDF: 7 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68600G (15 February 2008); doi: 10.1117/12.764264
Show Author Affiliations
Yuan Liu, National Taiwan Univ. (Taiwan)
Feng-Chieh Li, National Taiwan Univ. (Taiwan)
Hsiao-Chin Chen, National Taiwan Univ. Hospital (Taiwan)
Po-shou Chang, National Taiwan Univ. (Taiwan)
Shu-Mei Yang, National Taiwan Univ. Hospital (Taiwan)
Hsuan-Shu Lee, National Taiwan Univ. Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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