Share Email Print

Proceedings Paper

MRI-coupled spectrally resolved fluorescence tomography for in vivo imaging
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

A unique fluorescence imaging system incorporates multi-channel spectrometer-based optical detection directly into clinical MRI for simultaneous MR and spectrally-resolved fluorescence tomography acquisition in small animal and human breast-sized volumes. A custom designed MRI rodent coil adapted to accommodate optical fibers in a circular geometry for contact mode acquisition provides small animal imaging capabilities, and human breast-sized volumes are imaged using a clinical breast coil modified with an optical fiber patient array. Spectroscopy fibers couple light emitted from the tissue surface to sixteen highly sensitive CCD-based spectrometers operating in parallel. Tissue structural information obtained from standard and contrast enhanced T1-weighted images is used to spatially constrain the diffuse fluorescence tomography reconstruction algorithm, improving fluorescence imaging capabilities qualitatively and quantitatively. Simultaneous acquisition precludes the use of complex co-registration processes. Calibration procedures for the optical acquisition system are reviewed and the imaging limits of the system are investigated in homogeneous and heterogeneous gelatin phantoms containing Indocyanine Green (ICG). Prior knowledge of fluorescence emission spectra is used to de-couple fluorescence emission from residual excitation laser cross-talk. Preliminary in vivo data suggests improved fluorescence imaging in mouse brain tumors using MR-derived spatial priors. U-251 human gliomas were implanted intracranially into nude mice and combined contrast enhanced MRI/fluorescence tomography acquisition was completed at 24 hour intervals over the course of 72 hours after administration of an EGFR targeted NIR fluorophore. Reconstructed images demonstrate an inability to recover reasonable images of fluorescence activity without the use of MRI spatial priors.

Paper Details

Date Published: 7 March 2008
PDF: 9 pages
Proc. SPIE 6850, Multimodal Biomedical Imaging III, 68500K (7 March 2008); doi: 10.1117/12.764252
Show Author Affiliations
Scott C. Davis, Dartmouth College (United States)
Summer L. Gibbs-Strauss, Dartmouth College (United States)
Stephen B. Tuttle, Dartmouth College (United States)
Shudong Jiang, Dartmouth College (United States)
Roger Springett, Dartmouth College (United States)
Hamid Dehghani, Dartmouth College (United States)
Univ. of Exeter (United Kingdom)
Brian W. Pogue, Dartmouth College (United States)
Keith D. Paulsen, Dartmouth College (United States)

Published in SPIE Proceedings Vol. 6850:
Multimodal Biomedical Imaging III
Fred S. Azar; Xavier Intes, Editor(s)

© SPIE. Terms of Use
Back to Top