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Proceedings Paper

Multimodal optical microscopy for monitoring fast neuronal activity and signaling
Author(s): S. Pagès; I. Veilleux; P. De Koninck; D. Côté
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Paper Abstract

We present a video-rate optical microscope that allows simultaneous imaging of two-photon excited fluorescence (TPEF), second harmonic generation (SHG) and reflectance. The ms time resolution of the system together with its submicrometer spatial resolution make it an ideal tool for studying fast neuronal activity and signaling, to understand how action potentials are decoded molecularly. Transient trans-membrane potentials are measured with SHG, while the evoked calcium oscillations are monitored with TPEF. The ability of this system to monitor both signals simultaneously in multiple sub-compartments of living neurons should open the way to study how the electrical activity of neurons is encoded intracellularly.

Paper Details

Date Published: 15 February 2008
PDF: 8 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601X (15 February 2008); doi: 10.1117/12.764161
Show Author Affiliations
S. Pagès, Univ. Laval (Canada)
I. Veilleux, Univ. Laval (Canada)
P. De Koninck, Univ. Laval (Canada)
D. Côté, Univ. Laval (Canada)

Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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