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Proceedings Paper

Multi-spectral photo-acoustic molecular tomography resolves fluorochrome distribution with high resolution and sensitivity in small animals
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Paper Abstract

Current non-invasive imaging methods of fluorescent molecular probes in the visible and near-infrared suffer from low spatial resolution as a result of rapid light diffusion in biological tissues. We show that three-dimensional distribution of fluorochromes deep in small animals can be resolved with below 25 femtomole sensitivity and 150 microns spatial resolution by means of multi-spectral photoacoustic molecular tomography. The low sensitivity limit of the method is enabled by using the highly resonant absorption spectrum of a commonly used near-infrared fluorescent molecular probe Alexa Fluor® 750 in order to acquire differential images at multiple wavelengths with tomographic topology suitable for whole-body small animal imaging.

Paper Details

Date Published: 28 February 2008
PDF: 7 pages
Proc. SPIE 6856, Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, 685613 (28 February 2008); doi: 10.1117/12.764155
Show Author Affiliations
Daniel Razansky, Massachusetts General Hospital and Harvard Medical School (United States)
Technical Univ. of Munich and Helmholz Ctr. Munich (Germany)
Claudio Vinegoni, Massachusetts General Hospital and Harvard Medical School (United States)
Vasilis Ntziachristos, Massachusetts General Hospital and Harvard Medical School (United States)
Technical Univ. of Munich and Helmholz Ctr. Munich (Germany)


Published in SPIE Proceedings Vol. 6856:
Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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