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Proceedings Paper

Novel tissue phantom for testing a dual-modality diagnostic system: time-resolved fluorescence spectroscopy and high frequency ultrasound
Author(s): Yang Sun; Kuo-Chih Liao; Yinghua Sun; Jesung Park; Laura Marcu
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Paper Abstract

A unique tissue phantom is reported here that mimics the optical and acoustical properties of biological tissue and enables testing and validation of a dual-modality clinical diagnostic system combining time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) and ultrasound backscatter microscopy (UBM). The phantom consisted of contrast agents including silicon dioxide particles with a range of diameters from 0.5 to 10 μm acting as optical and acoustical scatterers, and FITC-conjugated dextran mimicking the endogenous fluorophore in tissue. The agents were encapsulated in a polymer bead attached to the end of an optical fiber with a 200 μm diameter using a UV-induced polymerization technique. A set of beads with fibers were then implanted into a gel-based matrix with controlled patterns including a design with lateral distribution and a design with successively changing depth. The configuration presented here allowed the validation of the hybrid fluorescence spectroscopic and ultrasonic system by detecting the lateral and depth distribution of the contrast agents, as well as for coregistration of the ultrasonic image with spectroscopic data. In addition, the depth of the beads in the gel matrix was changed to explore the effect of different concentration ratio of the mixture on the fluorescence signal emitted.

Paper Details

Date Published: 21 February 2008
PDF: 9 pages
Proc. SPIE 6870, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue, 68700D (21 February 2008); doi: 10.1117/12.764013
Show Author Affiliations
Yang Sun, Univ. of California, Davis (United States)
Kuo-Chih Liao, Univ. of California, Davis (United States)
Yinghua Sun, Univ. of California, Davis (United States)
Jesung Park, Univ. of California, Davis (United States)
Laura Marcu, Univ. of California, Davis (United States)


Published in SPIE Proceedings Vol. 6870:
Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue
Robert J. Nordstrom, Editor(s)

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