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Proceedings Paper

Laser scatter in clinical applications
Author(s): Ed Luther; William Geddie
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Paper Abstract

Brightfield Laser Scanning Imaging (BLSI) is available on Laser Scanning Cytometers (LSCs) from CompuCyte Corporation. Briefly, digitation of photodetector outputs is coordinated with the combined motions of a small diameter (typically 2 to 10 microns) laser beam scanning a specimen in the Y direction (directed by a galvanometer-driven scanning mirror) and the microscope stage motion in the X direction. The output measurements are assembled into a two-dimensional array to provide a "non-real" digital image, where each pixel value reports the amount of laser-scattered light that is obtained when the laser beam is centered on that location. Depending on the detector positions, these images are analogous to Differential Interference Contrast or Phase Contrast microscopy. We report the incorporation of the new laser scattering capabilities into the workflow of a high-volume clinical cytology laboratory at University Health Network, Toronto, Canada. The laboratory has been employing LSC technology since 2003 for immunophenotypic fluorescence analysis of approximately 1200 cytological specimens per year, using the Clatch methodology. The new BLSI component allows visualization of cellular morphology at higher resolution levels than is possible with standard brightfield microscopic evaluation of unstained cells. BLSI is incorporated into the triage phase, where evaluation of unstained samples is combined with fluorescence evaluation to obtain specimen background levels. Technical details of the imaging methodology will be presented, as well as illustrative examples from current studies and comparisons to detailed, but obscure, historical studies of cytology specimens based on phase contrast microscopy.

Paper Details

Date Published: 22 February 2008
PDF: 13 pages
Proc. SPIE 6864, Biomedical Applications of Light Scattering II, 686412 (22 February 2008); doi: 10.1117/12.763996
Show Author Affiliations
Ed Luther, CompuCyte Corp. (United States)
William Geddie, Univ. Health Network (Canada)

Published in SPIE Proceedings Vol. 6864:
Biomedical Applications of Light Scattering II
Adam Wax; Vadim Backman, Editor(s)

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