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Proceedings Paper

Ultrafast Yb:KGd(WO4)2 laser for multimodal biomedical imaging with reduced photodamage
Author(s): Arkady Major; Richard Cisek; Adam Tuer; Nicole Prent; Virginijus Barzda
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Paper Abstract

We report on the development and successful application of a femtosecond Yb:KGd(WO4)2 laser for multimodal imaging of various biological samples. Its operation at longer wavelengths, 1029 nm, provided efficient excitation and greatly reduced sample photobleaching. The laser produced ~300 fs pulses with up to 100 nJ of energy at 14.3 MHz repetition rate. This laser system enabled continuous imaging of various live samples for prolonged periods of time. The details of laser development and fluorescence imaging of isolated chloroplasts are reported.

Paper Details

Date Published: 7 February 2008
PDF: 7 pages
Proc. SPIE 6881, Commercial and Biomedical Applications of Ultrafast Lasers VIII, 688108 (7 February 2008); doi: 10.1117/12.763953
Show Author Affiliations
Arkady Major, Univ. of Manitoba (Canada)
Univ. of Toronto (Canada)
Richard Cisek, Univ. of Toronto (Canada)
Adam Tuer, Univ. of Toronto (Canada)
Nicole Prent, Univ. of Toronto (Canada)
Virginijus Barzda, Univ. of Toronto (Canada)


Published in SPIE Proceedings Vol. 6881:
Commercial and Biomedical Applications of Ultrafast Lasers VIII
Joseph Neev; Stefan Nolte; Alexander Heisterkamp; Christopher B. Schaffer, Editor(s)

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