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Proceedings Paper

Fluorescence correlation spectroscopy of single molecules on an optofluidic chip
Author(s): M. I. Rudenko; S. Kühn; E. J. Lunt; B. S. Phillips; D. W. Deamer; A. R. Hawkins; H. Schmidt
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Paper Abstract

We review our recent progress in bringing fluorescent correlation spectroscopy (FCS) of single molecules on a silicon optofluidic platform. Starting from basic concepts and applications of FCS we move to a description of our integrated optofluidic device, briefly outlining the physics behind its function and relevant geometrical characteristics. We then derive an FCS theoretical model for our sensor geometry, which we subsequently apply to the examination of molecular properties of single fluorophores and bioparticles. The model allows us to extract the diffusion coefficient, translational velocity and local concentration of particles in question. We conclude with future directions of this research.

Paper Details

Date Published: 13 February 2008
PDF: 10 pages
Proc. SPIE 6898, Silicon Photonics III, 689819 (13 February 2008); doi: 10.1117/12.763878
Show Author Affiliations
M. I. Rudenko, Univ. of California, Santa Cruz (United States)
S. Kühn, Univ. of California, Santa Cruz (United States)
E. J. Lunt, Brigham Young Univ. (United States)
B. S. Phillips, Brigham Young Univ. (United States)
D. W. Deamer, Univ. of California, Santa Cruz (United States)
A. R. Hawkins, Brigham Young Univ. (United States)
H. Schmidt, Univ. of California, Santa Cruz (United States)


Published in SPIE Proceedings Vol. 6898:
Silicon Photonics III
Joel A. Kubby; Graham T. Reed, Editor(s)

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