Share Email Print
cover

Proceedings Paper

Evaluating optical properties of isolated biological scatterers from confocal and low-coherence images
Author(s): David Levitz; Ravikant Samatham; Monica T. Hinds; Steven L. Jacques
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

In biomedical optics applications, the scattering of light by biological tissue is typically mimicked by embedding microparticles such as polystyrene microspheres or TiO2 within a non-scattering matrix. Such particles are well structured and give rise to uniform optical scattering properties. However, typical biological scatterers are seldom well-organized nor uniformly sized. In this work, we sought to characterize the scattering properties from particles common to many tissues such as collagen fibers, cells, and lipids. These purified particles were suspended and sandwiched between 2 glass cover slips to form disposable phantoms. The phantoms were imaged by optical coherence tomography and reflectance-mode confocal microscopy. From the images, the attenuation and reflectivity of the sample were evaluated by fitting the depth-dependent signal from specified regions of the image to a theoretical model. The fitted attenuation and reflectivity were used to deduce a distribution of local values of the scattering coefficient and anisotropy factor for each phantom. The measured optical properties at the 2 wavelengths differed in ways that can be explained by Mie theory, suggesting that despite their complex structure, typical biological scatterers exhibit some regularity that can potentially be characterized quantitatively.

Paper Details

Date Published: 21 February 2008
PDF: 5 pages
Proc. SPIE 6870, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue, 68700G (21 February 2008); doi: 10.1117/12.763776
Show Author Affiliations
David Levitz, Oregon Health and Science Univ. (United States)
Ravikant Samatham, Oregon Health and Science Univ. (United States)
Monica T. Hinds, Oregon Health and Science Univ. (United States)
Steven L. Jacques, Oregon Health and Science Univ. (United States)


Published in SPIE Proceedings Vol. 6870:
Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue
Robert J. Nordstrom, Editor(s)

© SPIE. Terms of Use
Back to Top