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Proceedings Paper

Combined chi (3) microscopy of the peripheral nervous tissue
Author(s): Christian P. Pfeffer; Martin Vogel; Bjorn R. Olsen; Feruz Ganikhanov
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Paper Abstract

We report an application of the combined third order microscopy techniques to reveal structure and morphology of the peripheral nerve in mice. The resonant Coherent Anti-Stokes Raman Scattering (CARS) and third harmonic generation (THG) techniques have been applied to visualize structure of the myelinated peripheral axon. While CARS was quite efficient in selective imaging of the cladding layer via characteristic Raman active vibrations of dense lipid structures constituting the layers, the THG microscopy helped to clearly reveal the degree of optical and nonlinear optical inhomogeneity of the axon core (that may have further important implications).

Paper Details

Date Published: 21 February 2008
PDF: 6 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 686010 (21 February 2008); doi: 10.1117/12.763743
Show Author Affiliations
Christian P. Pfeffer, Harvard Medical School (United States)
Martin Vogel, Harvard Univ. (United States)
Bjorn R. Olsen, Harvard Medical School (United States)
Feruz Ganikhanov, West Virginia Univ. (United States)

Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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