Share Email Print

Proceedings Paper

Fluorescence-enhanced europium complexes for the assessment of renal function
Author(s): Lori K. Chinen; Karen P. Galen; K. T. Kuan; Mary E. Dyszlewski; Hiroaki Ozaki; Hiroaki Sawai; Raghootama S. Pandurangi; Frederick G. Jacobs; Richard B. Dorshow; Raghavan Rajagopalan
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Real-time, non-invasive assessment of glomerular filtration rate (GFR) is essential not only for monitoring critically ill patients at the bedside, but also for staging and monitoring patients with chronic kidney disease. In our pursuit to develop exogenous luminescent probes for dynamic optical monitoring of GFR, we have prepared and evaluated Eu3+ complexes of several diethylenetriamine pentaacetate (DTPA)-monoamide ligands bearing molecular "antennae" to enhance metal fluorescence via the intramolecular ligand-metal fluorescence resonance energy transfer (FRET) process. The results show that Eu-DTPA-monoamide complex 13a, which contains a quinoxanlinyl antenna, exhibits large (c.a. 2700-fold) Eu3+ fluorescence enhancement over Eu-DTPA (4c). Indeed, complex 13a exhibits the highest fluorescent enhancement observed thus far in the DTPA-type metal complexes. The renal clearance profile of the corresponding radioactive 111In complex 13c is similar to that of 111In-DTPA, albeit 13c clears slower than 111In-DTPA. The biodistribution data indicates that 13c, and, by inference, 13a clear via a complex mechanism that includes glomerular filtration.

Paper Details

Date Published: 22 February 2008
PDF: 8 pages
Proc. SPIE 6867, Molecular Probes for Biomedical Applications II, 68670B (22 February 2008); doi: 10.1117/12.763665
Show Author Affiliations
Lori K. Chinen, Covidien (United States)
Karen P. Galen, Covidien (United States)
K. T. Kuan, Covidien (United States)
Mary E. Dyszlewski, Covidien (United States)
Hiroaki Ozaki, Gunma Univ. (Japan)
Hiroaki Sawai, Gunma Univ. (Japan)
Raghootama S. Pandurangi, Covidien (United States)
Frederick G. Jacobs, Covidien (United States)
Richard B. Dorshow, Covidien (United States)
Raghavan Rajagopalan, Covidien (United States)

Published in SPIE Proceedings Vol. 6867:
Molecular Probes for Biomedical Applications II
Samuel Achilefu; Darryl J. Bornhop; Ramesh Raghavachari, Editor(s)

© SPIE. Terms of Use
Back to Top