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Proceedings Paper

Imaging arterial cells, atherosclerosis, and restenosis by multimodal nonlinear optical microscopy
Author(s): Han-Wei Wang; Vlad Simianu; Matthew J. Locker; Michael Sturek; Ji-Xin Cheng
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Paper Abstract

By integrating sum-frequency generation (SFG), and two-photon excitation fluorescence (TPEF) on a coherent anti-Stokes Raman scattering (CARS) microscope platform, multimodal nonlinear optical (NLO) imaging of arteries and atherosclerotic lesions was demonstrated. CARS signals arising from CH2-rich membranes allowed visualization of endothelial cells and smooth muscle cells in a carotid artery. Additionally, CARS microscopy allowed vibrational imaging of elastin and collagen fibrils which are rich in CH2 bonds in their cross-linking residues. The extracellular matrix organization was further confirmed by TPEF signals arising from elastin's autofluorescence and SFG signals arising from collagen fibrils' non-centrosymmetric structure. The system is capable of identifying different atherosclerotic lesion stages with sub-cellular resolution. The stages of atherosclerosis, such as macrophage infiltration, lipid-laden foam cell accumulation, extracellular lipid distribution, fibrous tissue deposition, plaque establishment, and formation of other complicated lesions could be viewed by our multimodal CARS microscope. Collagen percentages in the region adjacent to coronary artery stents were resolved. High correlation between NLO and histology imaging evidenced the validity of the NLO imaging. The capability of imaging significant components of an arterial wall and distinctive stages of atherosclerosis in a label-free manner suggests the potential application of multimodal nonlinear optical microscopy to monitor the onset and progression of arterial diseases.

Paper Details

Date Published: 15 February 2008
PDF: 12 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68600W (15 February 2008); doi: 10.1117/12.763604
Show Author Affiliations
Han-Wei Wang, Purdue Univ. (United States)
Vlad Simianu, Indiana Univ. Medical School (United States)
Matthew J. Locker, Indiana Univ. Medical School (United States)
Michael Sturek, Indiana Univ. Medical School (United States)
Ji-Xin Cheng, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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