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Proceedings Paper

Cell-population tracking using quantum dots in flow cytometry
Author(s): Huw D. Summers; Rachel J. Errington; Paul J. Smith; Sally Chappell; Paul Rees; Martyn R. Brown; James F. Leary
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Paper Abstract

We have used flow-cytometry together with computational modeling of quantum dot portioning during cell division to identify population distributions of proliferating cells. The objective has been to develop a robust assay of integrated cellular fluorescence which reports the extent of cellular bifurcation within a complex population and potentially provides profiles of drug resistance, cell clonality and levels of aneuploidy in tumour cells. The implementation of a data analysis program based on genetic algorithms provides a complete description of the proliferation dynamics and gives values for the inter-mitotic time, the partitioning ratio of quantum dots between daughter cells and their associated deviations.

Paper Details

Date Published: 22 February 2008
PDF: 8 pages
Proc. SPIE 6865, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications V, 68650L (22 February 2008); doi: 10.1117/12.763602
Show Author Affiliations
Huw D. Summers, Cardiff Univ. (United Kingdom)
Rachel J. Errington, Cardiff Univ. (United Kingdom)
Paul J. Smith, Cardiff Univ. (United Kingdom)
Sally Chappell, Cardiff Univ. (United Kingdom)
Paul Rees, Swansea Univ. (United Kingdom)
Martyn R. Brown, Swansea Univ. (United Kingdom)
James F. Leary, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 6865:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications V
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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