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Proceedings Paper

Fluorescence lifetime estimation of multiple near-infrared dyes in mice
Author(s): Guobin Ma; Simon Fortier; Muriel Jean-Jacques; Niculae Mincu; Frederic Leblond; Zahia Ichalalene; Anader Benyamin-Seeyar; Mario Khayat
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Paper Abstract

In order to precisely recover fluorescence lifetimes from bulk tissues, one needs to employ complex light propagation models (e.g., the radiative transfer equation or a simpler yet consistent approximation, the diffusion equation) requiring knowledge of the tissue optical properties. This can be computationally expensive and therefore not practical in many applications. We present a novel method to estimate the fluorescence lifetimes of multiple fluorophores embedded in mice. By assuming that the photon diffusion does not significantly change the fluorescence decay slope, the light propagation is simply modeled as a time-delay during lifetime estimation. Applications of this approach are demonstrated by simulation, phantom data, and in vivo experiments.

Paper Details

Date Published: 12 February 2008
PDF: 8 pages
Proc. SPIE 6850, Multimodal Biomedical Imaging III, 685003 (12 February 2008); doi: 10.1117/12.763544
Show Author Affiliations
Guobin Ma, ART Advanced Research Technologies Inc. (Canada)
Simon Fortier, ART Advanced Research Technologies Inc. (Canada)
Muriel Jean-Jacques, ART Advanced Research Technologies Inc. (Canada)
Niculae Mincu, ART Advanced Research Technologies Inc. (Canada)
Frederic Leblond, ART Advanced Research Technologies Inc. (Canada)
Zahia Ichalalene, ART Advanced Research Technologies Inc. (Canada)
Anader Benyamin-Seeyar, ART Advanced Research Technologies Inc. (Canada)
Mario Khayat, ART Advanced Research Technologies Inc. (Canada)


Published in SPIE Proceedings Vol. 6850:
Multimodal Biomedical Imaging III
Fred S. Azar; Xavier Intes, Editor(s)

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