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Proceedings Paper

Time-dependent diffuse reflectance spectroscopy for in vivo characterization of pediatric epileptogenic brain lesions
Author(s): Sanghoon Oh; John Ragheb; Sanjiv Bhatia; David Sandberg; Mahlon Johnson; Bradley Fernald; Wei-Chiang Lin
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Paper Abstract

Optical spectroscopy for in vivo tissue diagnosis is performed traditionally in a static manner; a snap shot of the tissue biochemical and morphological characteristics is captured through the interaction between light and the tissue. This approach does not capture the dynamic nature of a living organ, which is critical to the studies of brain disorders such as epilepsy. Therefore, a time-dependent diffuse reflectance spectroscopy system with a fiber-optic probe was designed and developed. The system was designed to acquire broadband diffuse reflectance spectra (240 ~ 932 nm) at an acquisition rate of 33 Hz. The broadband spectral acquisition feature allows simultaneous monitoring of various physiological characteristics of tissues. The utility of such a system in guiding pediatric epilepsy surgery was tested in a pilot clinical study including 13 epilepsy patients and seven brain tumor patients. The control patients were children undergoing suregery for brain tumors in which measurements were taken from normal brain exposed during the surgery. Diffuse reflectance spectra were acquired for 12 seconds from various parts of the brain of the patients during surgery. Recorded spectra were processed and analyzed in both spectral and time domains to gain insights into the dynamic changes in, for example, hemodynamics of the investigated brain tissue. One finding from this pilot study is that unsynchronized alterations in local blood oxygenation and local blood volume were observed in epileptogenic cortex. These study results suggest the advantage of using a time-dependent diffuse reflectance spectroscopy system to study epileptogenic brain in vivo.

Paper Details

Date Published: 26 February 2008
PDF: 10 pages
Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 68422M (26 February 2008); doi: 10.1117/12.763407
Show Author Affiliations
Sanghoon Oh, Miami Children's Hospital (United States)
Florida International Univ. (United States)
John Ragheb, Miami Children's Hospital (United States)
Sanjiv Bhatia, Miami Children's Hospital (United States)
David Sandberg, Miami Children's Hospital (United States)
Mahlon Johnson, Univ. of Rochester Medical Ctr. (United States)
Bradley Fernald, Florida International Univ. (United States)
Wei-Chiang Lin, Miami Children's Hospital (United States)
Florida International Univ. (United States)


Published in SPIE Proceedings Vol. 6842:
Photonic Therapeutics and Diagnostics IV
Kenton W. Gregory; Guillermo J. Tearney; Reza S. Malek; Nikiforos Kollias; Bernard Choi; Haishan Zeng; Brian Jet-Fei Wong; Justus F. R. Ilgner; Henry Hirschberg; Steen J. Madsen, Editor(s)

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