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Proceedings Paper

Polarization dependant in vivo second harmonic generation imaging of Caenorhabditis elegans vulval, pharynx, and body wall muscles
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Paper Abstract

Second harmonic generation (SHG) imaging has emerged in recent years as an important laboratory imaging technique since it can provide unique structural information with submicron resolution. It enjoys the benefits of non-invasive interaction establishing this imaging modality as ideal for in vivo investigation of tissue architectures. In this study we present, polarization dependant high resolution SHG images of Caenorhabditis elegans muscles in vivo. We imaged a variety of muscular structures such as body walls, pharynx and vulva. By fitting the experimental data into a cylindrical symmetry spatial model we mapped the corresponding signal distribution of the χ(2) tensor and identified its main axis orientation for different sarcomeres of the earth worm. The cylindrical symmetry was considered to arise from the thick filaments architecture of the inside active volume. Moreover, our theoretical analysis allowed calculating the mean orientation of harmonophores (myosin helical pitch). Ultimately, we recorded and analysed vulvae muscle dynamics, where SHG signal decreased during in vivo contraction.

Paper Details

Date Published: 15 February 2008
PDF: 10 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 686008 (15 February 2008); doi: 10.1117/12.763270
Show Author Affiliations
Sotiris Psilodimitrakopoulos, Institut de Ciències Fotòniques (Spain)
Susana Santos, Institut de Ciències Fotòniques (Spain)
Ivan Amat-Roldan, Institut de Ciències Fotòniques (Spain)
Manoj Mathew, Institut de Ciències Fotòniques (Spain)
Anisha Thayil K. N., Institut de Ciències Fotòniques (Spain)
David Artigas, Univ. Politècnica de Catalunya (Spain)
Pablo Loza-Alvarez, Institut de Ciències Fotòniques (Spain)


Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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