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Proceedings Paper

Mucosal layer as major source of green autofluorescence in the colon under excitation by blue light
Author(s): Keimei Nakano; Yoshinori Harada; Yoshihisa Yamaoka; Kiichiro Miyawaki; Naoki Wakabayashi; Shoji Mitsufuji; Katsuichi Imaizumi; Hideyuki Takaoka; Masaya Nakaoka; Tetsuro Takamatsu
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Paper Abstract

Autofluorescence endoscopy is a promising modality for diagnosis of colonic tumors. This article discusses the origin of autofluorescence of the normal colon. Excised normal colons were analyzed by using fluorescence stereomicroscopy and a fluorescence-lifetime microscopy system. Fluorescence images showed that the mucosa had stronger autofluorescence than the submucosa. The results of fluorescence-lifetime measurement showed that nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) might be responsible for the autofluorescence of the colonic epithelia. Our results suggest that the mucosal autofluorescence generates by NADH and FAD was an important source of the green autofluorescence.

Paper Details

Date Published: 8 February 2008
PDF: 6 pages
Proc. SPIE 6853, Biomedical Optical Spectroscopy, 68531H (8 February 2008); doi: 10.1117/12.763245
Show Author Affiliations
Keimei Nakano, Kyoto Prefectural Univ. of Medicine (Japan)
Yoshinori Harada, Kyoto Prefectural Univ. of Medicine (Japan)
Yoshihisa Yamaoka, Kyoto Prefectural Univ. of Medicine (Japan)
Kiichiro Miyawaki, Kyoto Prefectural Univ. of Medicine (Japan)
Naoki Wakabayashi, Kyoto Prefectural Univ. of Medicine (Japan)
Shoji Mitsufuji, Kyoto Prefectural Univ. of Medicine (Japan)
Katsuichi Imaizumi, Olympus Medical Systems Corp. (Japan)
Hideyuki Takaoka, Olympus Corp. (Japan)
Masaya Nakaoka, Olympus Corp. (Japan)
Tetsuro Takamatsu, Kyoto Prefectural Univ. of Medicine (Japan)

Published in SPIE Proceedings Vol. 6853:
Biomedical Optical Spectroscopy
Anita Mahadevan-Jansen; Wolfgang Petrich; Robert R. Alfano; Alvin Katz, Editor(s)

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