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Imaging of anticancer agent distribution by a slit-scanning Raman microscope
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Paper Abstract

In recent years, various types of molecular imaging technologies have been developed, but many of them require probes and may have some influence on the distribution of the target molecules. In contrast, Raman microscopic analysis is effective for molecular identification of materials, and molecular imaging methods employing Raman scattering light can be applied to living organisms without use of any exogenous probes. Unfortunately, Raman microscopic imaging is rarely used in the biomedical field due to the weakness of Raman signals. When the conventional Raman microscopes are used, the acquisition of an image of a cell usually takes several hours. Recently, a slit-scanning confocal Raman microscope has been developed. It can acquire images of living cells and tissues with faster scanning speed. In this study, we used the slit-scanning confocal Raman microscope (RAMAN-11) to image the distribution of a drug in living cells. We could acquire images of the distribution of an anticancer reagent in living cells within several minutes. Since the wavelength of Raman scattering light is determined by the frequency of molecular vibration, the in situ mapping of the intracellular drugs without use of a probe is possible, suggesting that laser Raman imaging is a useful method for a variety of pharmacokinetic studies.

Paper Details

Date Published: 8 February 2008
PDF: 5 pages
Proc. SPIE 6853, Biomedical Optical Spectroscopy, 685308 (8 February 2008); doi: 10.1117/12.763078
Show Author Affiliations
Yoshinori Harada, Kyoto Prefectural Univ. of Medicine (Japan)
Taisuke Ota, Osaka Univ. (Japan)
Dai Ping, Kyoto Prefectural Univ. of Medicine (Japan)
Yoshihisa Yamaoka, Kyoto Prefectural Univ. of Medicine (Japan)
Keisaku Hamada, Osaka Univ. (Japan)
Katsumasa Fujita, Osaka Univ. (Japan)
Tetsuro Takamatsu, Kyoto Prefectural Univ. of Medicine (Japan)

Published in SPIE Proceedings Vol. 6853:
Biomedical Optical Spectroscopy
Anita Mahadevan-Jansen; Wolfgang Petrich; Robert R. Alfano; Alvin Katz, Editor(s)

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