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Proceedings Paper

Serotonin: multiphoton imaging and relevant spectral data
Author(s): S. K. Kaushalya; Suman Nag; J. Balaji; S. Maiti
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Paper Abstract

Coupling three-photon microscopy with automated stage movement can now produce a live high resolution map of the neurotransmitter serotonin in a single cross section of the whole rat brain. Accurate quantification of these serotonin images demands appropriate spectral filtering. This requires one to consider that the spectral characteristics of serotonin show a remarkable variation as it non-covalently associates with different molecules, as we discuss here. Also it is known that serotonin emission changes when it forms a covalent adduct with para-formaldehyde. This provides a potential route for producing a whole brain serotonin map using multiphoton microscopy in a fixed rat brain. Here we take the initial step showing that multiphoton microscopy of this adduct can quantitatively image chemically induced changes in serotonin distribution.

Paper Details

Date Published: 15 February 2008
PDF: 8 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601C (15 February 2008); doi: 10.1117/12.763020
Show Author Affiliations
S. K. Kaushalya, Tata Institute of Fundamental Research (India)
Suman Nag, Tata Institute of Fundamental Research (India)
J. Balaji, Univ. of California, Los Angeles (United States)
S. Maiti, Tata Institute of Fundamental Research (India)


Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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