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Proceedings Paper

Suppressing nonspecific adsorption of proteins on the single-molecular level
Author(s): Nicole Marmé; Haemi Lee; Achim Friedrich; Chong-Woo Park; Julie Fiore; David J. Nesbitt; Jens-Peter Knemeyer
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Paper Abstract

Avoiding nonspecific surface adsorption is a crucial and often challenging issue in many single-molecule studies and analytical applications. In this work, we investigated glass surfaces coated with cross-linking star-shaped polyethylene glycol (4-arm PEG) and demonstrated that this coating can be used for effective suppression of nonspecific protein binding, such as streptavidin. Single-molecule fluorescence images show that only a few molecules remain nonspecifically bound to surfaces treated with protein after sufficient rinsing, i.e. less than to a state-of-the-art BSA coating. Furthermore, different applications for star-shaped PEG-passivated surfaces are shown.

Paper Details

Date Published: 21 February 2008
PDF: 7 pages
Proc. SPIE 6862, Single Molecule Spectroscopy and Imaging, 686212 (21 February 2008); doi: 10.1117/12.763014
Show Author Affiliations
Nicole Marmé, JILA, National Institute of Standards and Technology (United States)
Univ. of Colorado (United States)
Univ. of Heidelberg (Germany)
Haemi Lee, JILA, National Institute of Standards and Technology (United States)
Univ. of Colorado (United States)
Achim Friedrich, German Cancer Research Ctr. (Germany)
Chong-Woo Park, Univ. of Heidelberg (Germany)
Julie Fiore, JILA, National Institute of Standards and Technology (United States)
Univ. of Colorado (United States)
David J. Nesbitt, JILA, National Institute of Standards and Technology (United States)
Univ. of Colorado (United States)
Jens-Peter Knemeyer, JILA, National Institute of Standards and Technology (United States)
Univ. of Colorado (United States)
German Cancer Research Ctr. (Germany)


Published in SPIE Proceedings Vol. 6862:
Single Molecule Spectroscopy and Imaging
Jörg Enderlein; Zygmunt K. Gryczynski; Rainer Erdmann, Editor(s)

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