Share Email Print
cover

Proceedings Paper

Noninvasive murine glioma detection improved following photobleaching of skin PpIX fluorescence
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Aminolevulinic Acid (ALA) is a prodrug which can be administered to cells, animals or patients after which it is transformed via the Heme synthesis pathway into the fluorescent molecule Protoporphyrin IX (PpIX). PpIX has been shown to be useful as both a photosensitizer for photodynamic therapy (PDT) and as a fluorescence imaging contrast agent. The ALA-PpIX system not only provides contrast for fluorescence imaging but also gives information about the metabolic activity of the imaged tissue and thus could be useful for monitoring cancer therapy. In the current study skin photobleaching was examined to determine if PpIX fluorescence contrast in malignant brain tumors could be better visualized noninvasively. Red light photobleaching decreased skin PpIX fluorescence and increased the ability to noninvasively quantify PpIX fluorescence in murine gliomas, as in vivo measurements of mean PpIX fluorescence more closely matched ex vivo quantification following skin photobleaching. Three doses of blue light photobleaching (4 J/cm2, 8 J/cm2 and 12 J/cm2) were tested and determined to give similar levels of skin photobleaching as well as a similar window of decreased skin PpIX fluorescence for noninvasive fluorescence imaging following the photobleaching dose administration.

Paper Details

Date Published: 25 February 2008
PDF: 9 pages
Proc. SPIE 6845, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVII, 68450D (25 February 2008); doi: 10.1117/12.762997
Show Author Affiliations
Summer L. Gibbs-Strauss, Dartmouth College (United States)
Scott C. Davis, Dartmouth College (United States)
Julia A. O'Hara, Dartmouth College (United States)
P. Jack Hoopes, Dartmouth College (United States)
Tayyaba Hasan, Wellman Center for Photomedicine, Massachusetts General Hospital (United States)
Brian W. Pogue, Dartmouth College (United States)
Wellman Center for Photomedicine, Massachusetts General Hospital (United States)


Published in SPIE Proceedings Vol. 6845:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVII
David Kessel, Editor(s)

© SPIE. Terms of Use
Back to Top