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Proceedings Paper

Non-invasive analysis/diagnosis of human normal and melanoma skin tissues with two-photon FLIM in vivo
Author(s): Iris Riemann; Alexander Ehlers; Ronan Le Harzic; Enrico Dimitrow; Martin Kaatz; P. Elsner; Rainer Bückle; Karsten König
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Paper Abstract

Multiphoton optical tomography provides non-invasive optical sectioning of skin with high spatial and intracellular resolution. The imaging system DermaInspect based on femtosecond laser pulses and equipped with a SPC 830 card for FLIM analysis was used to perform this technique in vivo in clinical studies on patients with abnormal pigmented tissues. After examination in vivo, biopsies were taken and analyzed again. So far it was possible to distinguish between normal and diseased tissues mainly due to their different morphological appearances in the imaging mode: melanoma tissues show differences in the structure of the epidermal layers and the presence of dendritic cells compared to normal skin. The optical analysis can be improved when using the system in the time-correlated single photon counting mode. Fluorescence lifetime analysis reveals characteristic differences between healthy and melanoma tissues even before the visible morphological changes in human tissue in vivo.

Paper Details

Date Published: 8 February 2008
PDF: 5 pages
Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 684205 (8 February 2008); doi: 10.1117/12.762937
Show Author Affiliations
Iris Riemann, Fraunhofer Institute of Biomedical Technology (Germany)
Alexander Ehlers, Fraunhofer Institute of Biomedical Technology (Germany)
Saarland Univ. (Germany)
Ronan Le Harzic, Fraunhofer Institute of Biomedical Technology (Germany)
Enrico Dimitrow, Univ. Jena (Germany)
Martin Kaatz, Univ. Jena (Germany)
P. Elsner, Univ. Jena (Germany)
Rainer Bückle, JenLab GmbH (Germany)
Karsten König, Fraunhofer Institute of Biomedical Technology (Germany)
Saarland Univ. (Germany)


Published in SPIE Proceedings Vol. 6842:
Photonic Therapeutics and Diagnostics IV
Kenton W. Gregory; Guillermo J. Tearney; Reza S. Malek; Nikiforos Kollias; Bernard Choi; Haishan Zeng; Brian Jet-Fei Wong; Justus F. R. Ilgner; Henry Hirschberg; Steen J. Madsen, Editor(s)

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