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Proceedings Paper

Two-photon imaging of stem cells
Author(s): A. Uchugonova; E. Gorjup; I. Riemann; D. Sauer; K. König
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Paper Abstract

A variety of human and animal stem cells (rat and human adult pancreatic stem cells, salivary gland stem cells, dental pulpa stem cells) have been investigated by femtosecond laser 5D two-photon microscopy. Autofluorescence and second harmonic generation have been imaged with submicron spatial resolution, 270 ps temporal resolution, and 10 nm spectral resolution. In particular, NADH and flavoprotein fluorescence was detected in stem cells. Major emission peaks at 460nm and 530nm with typical mean fluorescence lifetimes of 1.8 ns and 2.0 ns, respectively, were measured using time-correlated single photon counting and spectral imaging. Differentiated stem cells produced the extracellular matrix protein collagen which was detected by SHG signals at 435 nm.

Paper Details

Date Published: 15 February 2008
PDF: 10 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601W (15 February 2008); doi: 10.1117/12.762734
Show Author Affiliations
A. Uchugonova, Fraunhofer Institute of Biomedical Technology (Germany)
Saarland Univ. (Germany)
E. Gorjup, Fraunhofer Institute of Biomedical Technology (Germany)
I. Riemann, Fraunhofer Institute of Biomedical Technology (Germany)
D. Sauer, Fraunhofer Institute of Biomedical Technology (Germany)
K. König, Fraunhofer Institute of Biomedical Technology (Germany)
Saarland Univ. (Germany)


Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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