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Proceedings Paper

Characterization of dermal structural assembly in normal and pathological connective tissues by intrinsic signal multiphoton optical microscopy
Author(s): Julia G. Lyubovitsky; Xiaoman Xu; Chung-ho Sun; Bogi Andersen; Tatiana B. Krasieva; Bruce J. Tromberg
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Paper Abstract

Employing a reflectance multi-photon microscopy (MPM) technique, we developed novel method to quantitatively study the three-dimensional assembly of structural proteins within bulk of dermal ECMs. Using a structurally simplified model of skin with enzymatically dissected epidermis, we find that low resolution MPM clearly discriminates between normal and pathological dermis. High-resolution images revealed that the backscattered MPM signals are affected by the assembly of collagen fibrils and fibers within this system. Exposure of tissues to high concentrations of potentially denaturing chemicals also resulted in the reduction of SHG signals from structural proteins which coincided with the appearance of aggregated fluorescent structures.

Paper Details

Date Published: 7 February 2008
PDF: 9 pages
Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 685902 (7 February 2008); doi: 10.1117/12.762702
Show Author Affiliations
Julia G. Lyubovitsky, Univ. of California, Riverside (United States)
Xiaoman Xu, Univ. of California, Irvine (United States)
Chung-ho Sun, Beckman Laser Institute, Univ. of California, Irvine (United States)
Bogi Andersen, Univ. of California, Irvine (United States)
Tatiana B. Krasieva, Beckman Laser Institute, Univ. of California, Irvine (United States)
Bruce J. Tromberg, Beckman Laser Institute, Univ. of California, Irvine (United States)
Univ. of California, Irvine (United States)


Published in SPIE Proceedings Vol. 6859:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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