Share Email Print
cover

Proceedings Paper

Femtosecond laser manipulation techniques for individual patterning of biological micro-object
Author(s): Yoichiroh Hosokawa; Yuqiang Jiang; Isamu Oh; Noriko Takizawa; Takayuki Uwada; Kazunori Okano; Hiroshi Masuhara
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Several kinds of manipulation of biological cells were performed utilizing regeneratively amplified femtosecond laser system. When single-shot pulse of an amplified Ti: Sapphire femtosecond laser pulse is focused on a culture medium, shockwave and cavitation bubble are generated with little heating. An impulsive force resulting in these phenomena was applied to pttern specific cells form a culture substrate. Furthermore, laser trapping of cells was realized using high-repetition rate pulses from the laser oscillator. Although the cell was trapped stably when the laser power was less than 100 mW, the cell was burst above the threshold laser power. The bursting would be due to heating inside cell, on which the laser was focused and multiphoton absorption was induced. On the bases of these results, we propose a new methodology to pattern biological cells, which is speedy and flexible when compared with previous micropatterning methods.

Paper Details

Date Published: 20 February 2008
PDF: 6 pages
Proc. SPIE 6854, Optical Interactions with Tissue and Cells XIX, 68541K (20 February 2008); doi: 10.1117/12.762605
Show Author Affiliations
Yoichiroh Hosokawa, Hamano Life Science Research Foundation (Japan)
Osaka Univ. (Japan)
CREST, Japan Science and Technology Agency (Japan)
Yuqiang Jiang, Hamano Life Science Research Foundation (Japan)
Handai Nano Photonics Institute (Japan)
Isamu Oh, Handai Nano Photonics Institute (Japan)
Osaka Univ. (Japan)
Noriko Takizawa, Hamano Life Science Research Foundation (Japan)
Takayuki Uwada, Hamano Life Science Research Foundation (Japan)
Kazunori Okano, Hamano Life Science Research Foundation (Japan)
Osaka Univ. (Japan)
Hiroshi Masuhara, Hamano Life Science Research Foundation (Japan)
Handai Nano Photonics Institute (Japan)
Osaka Univ. (Japan)


Published in SPIE Proceedings Vol. 6854:
Optical Interactions with Tissue and Cells XIX
Steven L. Jacques; William P. Roach; Robert J. Thomas, Editor(s)

© SPIE. Terms of Use
Back to Top