Share Email Print
cover

Proceedings Paper

Viability evaluation of culture cells patterned by femtosecond laser-induced impulsive force
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

PC12 cells, which are derived from a rat pheochromocytoma, were independently patterned utilizing an impulsive force resulting in impulsive shockwave and cavitation bubble generation by focused femtosecond laser irradiation. Since the PC12 cells respond reversibly to nerve growth factor by induction of the neuronal phenotype, we can assess an influence that the impulsive force gives to the bioactivity in term of the cell differentiation. The patterned cells were accumulated on an intact dish and cultured for 3 days. The behavior of appearance and cell differentiation was observed by multipoint time-lapse system. On bases of these results, it was proved that the biological activity of the cell is unaffected by the femtosecond laser patterning.

Paper Details

Date Published: 20 February 2008
PDF: 6 pages
Proc. SPIE 6854, Optical Interactions with Tissue and Cells XIX, 685411 (20 February 2008); doi: 10.1117/12.762584
Show Author Affiliations
Noriko Takizawa, Hamano Life Science Research Foundation (Japan)
Kazunori Okano, Hamano Life Science Research Foundation (Japan)
Osaka Univ. (Japan)
Takayuki Uwada, Hamano Life Science Research Foundation (Japan)
Yoichiroh Hosokawa, Hamano Life Science Research Foundation (Japan)
Osaka Univ. (Japan)
CREST, Japan Science and Technology Agency (Japan)
Hiroshi Masuhara, Hamano Life Science Research Foundation (Japan)
Osaka Univ. (Japan)
CREST, Japan Science and Technology Agency (Japan)


Published in SPIE Proceedings Vol. 6854:
Optical Interactions with Tissue and Cells XIX
Steven L. Jacques; William P. Roach; Robert J. Thomas, Editor(s)

© SPIE. Terms of Use
Back to Top