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Proceedings Paper

Studying cell dynamics and function with CLASS microscopy
Author(s): Le Qiu; Edward Vitkin; Saira Salahuddin; Bradley S. Turner; Sarah E. Keates; Mark D. Modell; Rama Bansil; Irving Itzkan; Eugene B. Hanlon; Lev T. Perelman
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Paper Abstract

Confocal light absorption and scattering spectroscopic (CLASS) microscopy is a novel optical technique for observing submicron intracellular structures in living cells. It allows monitoring nondestructively cell function and cell dynamics in vivo and in real time. CLASS microscopy, having accuracy well beyond the diffraction limit, does not require cell fixation as the electron microscopy. In addition, it provides not only size information but also information about the biochemical and physical properties of the cell. CLASS microscopy can also visualize multiple compartments inside of living cell without employing exogenous molecular markers which are required by fluorescence microscopy and which can affect normal cell functioning. Recently we improved our CLASS microscope by utilizing the full power output of the supercontinuum laser and used it to study apoptosis in live cells.

Paper Details

Date Published: 11 March 2008
PDF: 8 pages
Proc. SPIE 6864, Biomedical Applications of Light Scattering II, 68640T (11 March 2008); doi: 10.1117/12.762251
Show Author Affiliations
Le Qiu, Beth Israel Deaconess Medical Ctr., Harvard Univ. (United States)
Edward Vitkin, Beth Israel Deaconess Medical Ctr., Harvard Univ. (United States)
Saira Salahuddin, Beth Israel Deaconess Medical Ctr., Harvard Univ. (United States)
Bradley S. Turner, Beth Israel Deaconess Medical Ctr., Harvard Univ. (United States)
Sarah E. Keates, Beth Israel Deaconess Medical Ctr., Harvard Univ. (United States)
Mark D. Modell, Beth Israel Deaconess Medical Ctr., Harvard Univ. (United States)
Rama Bansil, Boston Univ. (United States)
Irving Itzkan, Beth Israel Deaconess Medical Ctr., Harvard Univ. (United States)
Eugene B. Hanlon, Dept. of Veterans Affairs (United States)
Lev T. Perelman, Beth Israel Deaconess Medical Ctr., Harvard Univ. (United States)


Published in SPIE Proceedings Vol. 6864:
Biomedical Applications of Light Scattering II
Adam Wax; Vadim Backman, Editor(s)

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