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Proceedings Paper

Integrated Raman and angular scattering microscopy (IRAM)
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Paper Abstract

A microscope system has been constructed that allows simultaneous acquisition of Raman scattering spectra and elastic scattering Fourier-plane data. The Raman scattering channel reports on chemical composition of the microscopic sample while the elastic scattering channel reports on morphological information about the sample. The system has been validated by acquiring data from single polystyrene beads and analyzing the elastic scattering signal using Generalized Lorenz-Mie Theory while comparing the Raman scattering signature to other polystyrene spectra from the literature. Monocytes and neutrophils, two immune cell types, have also been studied and show clear chemical and morphological differences between cell types.

Paper Details

Date Published: 10 March 2008
PDF: 12 pages
Proc. SPIE 6853, Biomedical Optical Spectroscopy, 68530T (10 March 2008); doi: 10.1117/12.761796
Show Author Affiliations
Zachary J. Smith, Institute of Optics, Univ. of Rochester (United States)
Andrew J. Berger, Institute of Optics, Univ. of Rochester (United States)


Published in SPIE Proceedings Vol. 6853:
Biomedical Optical Spectroscopy
Anita Mahadevan-Jansen; Wolfgang Petrich; Robert R. Alfano; Alvin Katz, Editor(s)

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