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Proceedings Paper

Determination of 3D optic axis orientation in cartilage by polarization-sensitive optical coherence tomography
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Paper Abstract

Polarization-sensitive optical coherence tomography has been used to solve fast-axis fibre orientation in three dimension space. Previously we have demonstrated that the apparent variations in polar angle orientation of collagen fibers along sagittal ridge of equine third metacarpophalangeal joint exist. A quantitative method based on multiple angles of illumination has been proposed to determine the polar angle of the collagen fibers. This method however ignored the full 3D structure by assuming that the collagen fibers long-axis lay within the plane of incidence. A new quantitative method based on the theory of light propagation in uniaxial materials is described which avoids this assumption. To test this method we have performed control experiments on a sample of equine tendon (this tissue has well defined c-axis lying along the long-axis of the tendon). Several samples of tendon were cut to achieve a planar surface inclined at -20° to the long axis. Additional 30° rotation provided non-zero azimuthal angle. The surface was then imaged using incident beam angles -40°, -20°, 0, +20°, +40° in two orthogonal planes. Values for both the polar and azimuthal angles were then derived using a numerical optimisation procedure. Results agreed qualitatively with the nominal values but suggested that the accuracy was limited by our method of determining the apparent birefringence.

Paper Details

Date Published: 6 February 2008
PDF: 9 pages
Proc. SPIE 6858, Optics in Tissue Engineering and Regenerative Medicine II, 68580I (6 February 2008); doi: 10.1117/12.761766
Show Author Affiliations
Nadya Ugryumova, Univ. of Sheffield (United Kingdom)
Marco Bonesi, Univ. of Sheffield (United Kingdom)
Stephen J. Matcher, Univ. of Sheffield (United Kingdom)

Published in SPIE Proceedings Vol. 6858:
Optics in Tissue Engineering and Regenerative Medicine II
Sean J. Kirkpatrick; Ruikang K. Wang, Editor(s)

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