Share Email Print
cover

Proceedings Paper

In vivo photoacoustic imaging of nude mice vasculature using a photoacoustic imaging system based on a commercial ultrasound scanner
Author(s): Ladislav Jankovic; Khalid Shahzad; Yao Wang; Michael Burcher; Frank-Detlef Scholle; Peter Hauff; Sabine Mofina; Mihaela Skobe
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

In-vivo photoacoustic/ultrasound (PA/US) imaging of nude mice was investigated using a photoacoustic imaging system based on a commercial ultrasound scanner HDI-5000. Raw per-channel data was captured and beamformed to generate each individual photoacoustic image with a single laser shot. An ultra-broadband CL15-7 linear array with a center frequency of 8 MHz, combined with a Schott Glass fiber bundle, was used as a compact high resolution imaging probe, with lateral and axial PA resolutions of about 300µm and 200µm, respectively. The imaging system worked in a dual PA-US mode, with the ultrasound outlining the tissue structure and the photoacoustic image showing the blood vessels. PA signals were generated by exposing mice to ultra-short optical pulses from a Nd:YAG-pumped OPO laser operating in a wavelength range of 700-950nm. The corresponding ultrasound images were generated in the regular B-mode with standard delay-and-sum beamforming algorithm. The system resolution was sufficiently high to identify and clearly distinguish the dorsal artery and the two lateral veins in the mouse tail. Both the saphena artery and the ischiatic vein on the cross-section of the mouse leg were clearly outlined in the PA images and correctly overlaid on the ultrasound image of the tissue structure. Similarly, cross-section PA images of the mouse abdomen revealed mesenteric vasculatures located below the abdominal wall. Finally, a successful PA imaging of the mouse thoracic cavity unveiled the ascending and descending aorta. These initial results demonstrate a great potential for a dual photoacoustic/ultrasound imaging modality implemented on a commercial ultrasound imaging scanner.

Paper Details

Date Published: 4 March 2008
PDF: 12 pages
Proc. SPIE 6856, Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, 68560N (4 March 2008); doi: 10.1117/12.761740
Show Author Affiliations
Ladislav Jankovic, Philips Research NA (United States)
Khalid Shahzad, Philips Research NA (United States)
Yao Wang, Philips Research NA (United States)
Michael Burcher, Philips Research NA (United States)
Frank-Detlef Scholle, Bayer Schering Pharma AG (Germany)
Peter Hauff, Bayer Schering Pharma AG (Germany)
Sabine Mofina, Mount Sinai School of Medicine (United States)
Mihaela Skobe, Mount Sinai School of Medicine (United States)


Published in SPIE Proceedings Vol. 6856:
Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

© SPIE. Terms of Use
Back to Top