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Proceedings Paper

SLIM for multispectral FRET imaging
Author(s): A. Rück; F. Dolp; R. Steiner; C. Steinmetz; B. von Einem; C.A.F. von Arnim
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Paper Abstract

Spectral fluorescence lifetime imaging (SLIM) is an advanced imaging technique, which combines spectral with time resolved detection. Real spectral information is achieved by using a grating in front of a PML-array, which allows time-correlated single photon counting (TCSPC). Whereas spectrally resolved fluorescence imaging alone has a reasonable sensitivity, the specificity of fluorescence detection can be improved by considering the fluorescence lifetime. The various possibilities which SLIM offers to improve FRET (resonant energy transfer) will be discussed as well as successfully realized applications. These include FRET measurements for protein interactions, related to Alzheimer's disease. Special attention will be focused on molecules involved in the processing and trafficking of the amyloid precursor protein (APP), as trafficking proteins of the GGA family and β-secretase BACE). Taking into account also the lifetime of the acceptor could enhance reliability of the FRET result.

Paper Details

Date Published: 15 February 2008
PDF: 7 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601F (15 February 2008); doi: 10.1117/12.761660
Show Author Affiliations
A. Rück, Institute for Laser Technologies in Medicine and Metrology (Germany)
F. Dolp, Institute for Laser Technologies in Medicine and Metrology (Germany)
R. Steiner, Institute for Laser Technologies in Medicine and Metrology (Germany)
C. Steinmetz, Univ. Ulm (Germany)
B. von Einem, Univ. Ulm (Germany)
C.A.F. von Arnim, Univ. Ulm (Germany)


Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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