Share Email Print
cover

Proceedings Paper

Investigation of the mechanism of transdermal penetration enhancer: a comparison of multiphoton microscopy and electron microscopy
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The aim of this study is to characterize the ability of multiphoton microscopy in monitoring the transdermal penetration enhancing effect of a depilatory agent and the associated structural alterations of stratum corneum. The result is compared with the electron microscopic findings. Our results show that the penetration of both hydrophilic and hydrophobic agents can be enhanced. The morphology of corneocytes becomes a homogenized pattern with focal detachment of surface corneocytes. In combination with Nile red staining, multiphoton imaging also shows that the regular motar-like distribution of lipid matrix was disrupted into a homogenized pattern of lipid distribution. These results are well correlated with the findings of ultrastructural analysis by electron micrographs showing disintegration of the protein envelope of coenocytes, disruption of intracellular keratin and loss of the regular lamellar packing of intercellular lipids. We conclude that, in addition to quantifying the permeation profiles of model drugs, multiphoton microscopy is able to detect the penetration enhancer-induced structural alterations of stratum corneum.

Paper Details

Date Published: 8 February 2008
PDF: 10 pages
Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 684203 (8 February 2008); doi: 10.1117/12.761429
Show Author Affiliations
Sung-Jan Lin, National Taiwan Univ. (Taiwan)
National Taiwan Univ. Hospital and College of Medicine (Taiwan)
Jin-Ning Lee, National Taiwan Univ. (Taiwan)
Chiao-Ying Lin, National Taiwan Univ. (Taiwan)
Chih-Chieh Chan, National Taiwan Univ. (Taiwan)
National Taiwan Univ. Hospital and College of Medicine (Taiwan)
Ming-Gu Lin, National Taiwan Univ. (Taiwan)
Chun-Chin Wang, National Taiwan Univ. (Taiwan)
Hsin-Yuan Tan, National Taiwan Univ. (Taiwan)
Chang Gung Memorial Hospital (Taiwan)
Tsung-Hua Tsai, Far Eastern Memorial Hospital (Taiwan)
Shiou-Hwa Jee, National Taiwan Univ. Hospital and College of Medicine (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 6842:
Photonic Therapeutics and Diagnostics IV
Kenton W. Gregory; Guillermo J. Tearney; Reza S. Malek; Nikiforos Kollias; Bernard Choi; Haishan Zeng; Brian Jet-Fei Wong; Justus F. R. Ilgner; Henry Hirschberg; Steen J. Madsen, Editor(s)

© SPIE. Terms of Use
Back to Top