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Proceedings Paper

Latex immunoagglutination assay for bovine viral diarrhea virus utilizing forward light scattering in a microfluidic device
Author(s): Brian C. Heinze; Jae-Young Song; Jin-Hee Han; Jeong-Yeol Yoon
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Paper Abstract

We have investigated the utilization of particle agglutination assays using forward light scattering measurements in a microfluidic device towards detecting viral particles. The model viral target was bovine viral diarrhea virus (BVDV). Highly carboxylated polystyrene microspheres (510 nm) were coated with anti-BVDV monoclonal antibodies. This solution was in turn used to detect live modified BVDV. This assay was first performed in a two well slide for proof of concept and then in a simple y-channel microfluidic device with optical fibers arranged in a close proximity setup. Particle immunoagglutination was detected through static light scattering measurements taken at 45° to incident light. In the microfluidic device, modified live BVDV was detected with a detection limit of 0.5 TCID50 mL-1.

Paper Details

Date Published: 1 March 2008
PDF: 6 pages
Proc. SPIE 6886, Microfluidics, BioMEMS, and Medical Microsystems VI, 688605 (1 March 2008); doi: 10.1117/12.761341
Show Author Affiliations
Brian C. Heinze, The Univ. of Arizona (United States)
Jae-Young Song, National Veterinary Research and Quarantine Service (South Korea)
Jin-Hee Han, The Univ. of Arizona (United States)
Jeong-Yeol Yoon, The Univ. of Arizona (United States)

Published in SPIE Proceedings Vol. 6886:
Microfluidics, BioMEMS, and Medical Microsystems VI
Wanjun Wang; Claude Vauchier, Editor(s)

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