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Proceedings Paper

FLIM and FCS measurements performed with a master oscillator fiber amplifier (MOFA) laser at 530 nm
Author(s): Felix Koberling; Martin Langkopf; Dietmar Klemme; Andreas Bülter; Volker Buschmann; Kristian Lauritsen; Rainer Erdmann
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Paper Abstract

Upgrade kits towards time-resolved measurements for Confocal Microscopes allow new measurement modes like Fluorescence Lifetime Imaging (FLIM), time-resolved analysis of Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Resonance Energy Transfer (FRET). Microscope users would typically like to use the same excitation wavelength for time-resolved measurements as for steady-state measurements, because their fluorophores are designed for the CW-laser wavelengths usually provided with the system. Pulsed diode lasers, which are ideally used for these upgrade kits are, however, not available for every spectral region of interest. Especially for "green" excitation around 530 nm this is still a problem, as there are no direct emitting laser diodes available. We present a new picosecond pulsed laser system for 530 nm emission with variable repetition rate and pulse energy, which is ideally suited for time-resolved measurements using Time-Correlated Single Photon Counting (TCSPC), and demonstrate its integration into a confocal microscope as well as first results of FLIM and FCS measurements.

Paper Details

Date Published: 15 February 2008
PDF: 6 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68601N (15 February 2008); doi: 10.1117/12.761229
Show Author Affiliations
Felix Koberling, PicoQuant GmbH (Germany)
Martin Langkopf, PicoQuant GmbH (Germany)
Dietmar Klemme, PicoQuant GmbH (Germany)
Andreas Bülter, PicoQuant GmbH (Germany)
Volker Buschmann, PicoQuant GmbH (Germany)
Kristian Lauritsen, PicoQuant GmbH (Germany)
Rainer Erdmann, PicoQuant GmbH (Germany)

Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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