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Proceedings Paper

Coherent interaction of optical second harmonic generation in collagen fibrils
Author(s): Shi-Wei Chu; Shih-Peng Tai; Ming-Che Chan; Chi-Kuang Sun; I-Ching Hsiao; Chi-Hung Lin; Yung-Chih Chen; Bai-Ling Lin
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Paper Abstract

We present the first experimental comparison between optical second harmonic generation images and atomic force microscope images in a matrix of nano-scaled collagen fibrils. Substantial variation of forward and backward propagated second harmonic generation radiation is observed in a single collagen fibril and is nicely correlated with the accurately determined thickness from an atomic force microscope. Contradicting to conventional nonlinear optical theory, our result indicates a linear relationship between fibril thickness and forward / backward second harmonic generation ratio. This is the first demonstration of estimating fibril thickness with nanometer precision by a noninvasive optical method.

Paper Details

Date Published: 12 March 2008
PDF: 8 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 686004 (12 March 2008); doi: 10.1117/12.761033
Show Author Affiliations
Shi-Wei Chu, National Taiwan Univ. (Taiwan)
Shih-Peng Tai, National Taiwan Univ. (Taiwan)
Ming-Che Chan, National Taiwan Univ. (Taiwan)
Chi-Kuang Sun, National Taiwan Univ. (Taiwan)
Research Ctr. for Applied Sciences (Taiwan)
I-Ching Hsiao, National Yang-Ming Univ. (Taiwan)
Chi-Hung Lin, National Yang-Ming Univ. (Taiwan)
Yung-Chih Chen, Baker Heart Research Institute (Australia)
Bai-Ling Lin, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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