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Proceedings Paper

Development of fluorescent contrast agents for optical imaging of mouse disease models
Author(s): J. Kovar; M. Simpson; A. Schutz-Geschwender; X. Xu; W. M. Volcheck; E. Sevick-Muraca; D. M. Olive
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Paper Abstract

Optical imaging is a rapidly developing field of research aimed at non-invasively interrogating animals for disease progression, determining the effects of a drug on a particular pathology, assessing the pharmacokinetic behavior of a drug, or identifying molecular biomarkers of disease. One of the key components of molecular imaging is the development of specific, targeted imaging contrast agents to assess these biological processes. The development of robust fluorochrome-labeled optical agents is a process that is often underestimated in terms of its complexity. We describe here the development process and performance issues for three different optical agents: IRDye 800CW EGF (epidermal growth factor), IRDye(R) 800CW 2-DG (2-deoxy D-glucose), and an IRDye 680 BoneTagTM. In vitro competitive assays were developed for two of the markers to demonstrate specificity. Specificity was confirmed in animal studies. Uptake of IRDye 800CW 2-DG was also examined by near-infrared confocal microscopy. Histological examinations were performed on target and non-target tissues following the completion of the imaging studies. The issues unique to the development of each labeled marker are discussed.

Paper Details

Date Published: 22 February 2008
PDF: 8 pages
Proc. SPIE 6867, Molecular Probes for Biomedical Applications II, 686707 (22 February 2008); doi: 10.1117/12.760815
Show Author Affiliations
J. Kovar, LI-COR Biosciences (United States)
M. Simpson, Univ. of Nebraska (United States)
A. Schutz-Geschwender, LI-COR Biosciences (United States)
X. Xu, LI-COR Biosciences (United States)
W. M. Volcheck, LI-COR Biosciences (United States)
E. Sevick-Muraca, Baylor College of Medicine (United States)
D. M. Olive, LI-COR Biosciences (United States)

Published in SPIE Proceedings Vol. 6867:
Molecular Probes for Biomedical Applications II
Samuel Achilefu; Darryl J. Bornhop; Ramesh Raghavachari, Editor(s)

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