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Proceedings Paper

Least invasive in vivo imaging using harmonic generation microscopy
Author(s): Szu-Yu Chen; Shih-Peng Tai; Cho-Shuen Hsieh; Chao-Yu Chen; Che-Hang Yu; Yen-Wei Lee; Wen-Jeng Lee; Shee-Uan Chen; Fu-Hsiung Chang; Chi-Kuang Sun
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Paper Abstract

In this manuscript, we review the physics and recent developments of the least invasive optical higher harmonic generation microscopy, with an emphasis on the in vivo molecular imaging applications. Optical higher harmonicgenerations, including second harmonic generation (SHG) and third harmonic generation (THG), leave no energy deposition to the interacted matters due to their energy-conservation characteristic, providing the "noninvasiveness" nature desirable for clinical studies. Combined with their nonlinearity, harmonic generation microscopy provides threedimensional sectioning capability, offering new insights into live samples. By choosing the lasers working in the high penetration window, we have recently developed a least-invasive in vivo light microscopy with submicron 3D resolution and high penetration, utilizing endogenous and resonantly-enhanced multi-harmonic-generation signals in live specimens, with focused applications on the developmental biology study and clinical virtual biopsy.

Paper Details

Date Published: 5 February 2008
PDF: 11 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 686002 (5 February 2008); doi: 10.1117/12.760560
Show Author Affiliations
Szu-Yu Chen, National Taiwan Univ. (Taiwan)
Shih-Peng Tai, National Taiwan Univ. (Taiwan)
Cho-Shuen Hsieh, National Taiwan Univ. (Taiwan)
Chao-Yu Chen, National Taiwan Univ. (Taiwan)
Che-Hang Yu, National Taiwan Univ. (Taiwan)
Yen-Wei Lee, National Taiwan Univ. (Taiwan)
Wen-Jeng Lee, National Taiwan Univ. (Taiwan)
Shee-Uan Chen, National Taiwan Univ. (Taiwan)
Fu-Hsiung Chang, National Taiwan Univ. (Taiwan)
Chi-Kuang Sun, National Taiwan Univ. (Taiwan)
Academia Sinica (Taiwan)

Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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