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Proceedings Paper

Tissue morphology from spectral polarimetry
Author(s): Donald D. Duncan; James C. Gladdish; Brandon D. Markway
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Paper Abstract

An important issue in the development of cultured tissues is the alignment of the cells within the scaffold, or on the substrate. Proper alignment leads to optimum tissue strength and it has been demonstrated that proper alignment is engendered by application of physiologically realistic stresses during the cell proliferation process. In situ monitoring of cell alignment during development can provide important feedback information in determining the optimum stresses. Numerical calculations suggest that cell aspect and orientation can be inferred from the polarization of the light scattered by these cells. In this paper, we demonstrate that a measurement of the wavelength-dependent depolarization of the light scattered from the cell layer reveals the alignment of these cells. We present results of experimental measurements on human umbilical vein endothelial cells (HUVEC's) layered onto glass cover slips and of simulations using T-matrix methods.

Paper Details

Date Published: 6 February 2008
PDF: 8 pages
Proc. SPIE 6858, Optics in Tissue Engineering and Regenerative Medicine II, 685809 (6 February 2008); doi: 10.1117/12.760512
Show Author Affiliations
Donald D. Duncan, Oregon Health & Science Univ. (United States)
James C. Gladdish, Oregon Health & Science Univ. (United States)
Brandon D. Markway, Oregon Health & Science Univ. (United States)

Published in SPIE Proceedings Vol. 6858:
Optics in Tissue Engineering and Regenerative Medicine II
Sean J. Kirkpatrick; Ruikang K. Wang, Editor(s)

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