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Proceedings Paper

Raman spectroscopic study of a genetically altered kidney cell
Author(s): Joel Joshi; Francisco Garcia; Silvia P. Centeno; N. V. Joshi
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Paper Abstract

A Raman spectroscopic investigation of a genetically altered Human Embryonic Kidney Cell (HEK293) along with a pathologically normal cell has been carried out by a conventional method. The genetic alteration was carried out with a standard protocol by using a Green Fluorescence Protein (GFP). Raman spectra show that there are dramatic differences between the spectrum obtained from a genetically altered cell and that obtained from a pathologically normal cell. The former shows three broad bands; meanwhile the latter shows several sharp peaks corresponding to the ring vibrational modes of Phen, GFP and DNA. The present analysis provides an indication that the force field near Phen located at 64, 65 and 66 was altered during the genetic transformation. The Raman spectrum could be a direct experimental evidence for substantial modifications triggered due to the expression of specific genes.

Paper Details

Date Published: 5 February 2008
PDF: 9 pages
Proc. SPIE 6853, Biomedical Optical Spectroscopy, 685304 (5 February 2008); doi: 10.1117/12.760317
Show Author Affiliations
Joel Joshi, Hospital Virgen de Macarena (Spain)
Francisco Garcia, Univ. Hospital of Puerto Real, Univ. of Cadiz (Spain)
Silvia P. Centeno, Univ. of Malaga (Spain)
N. V. Joshi, Univ. of Los Andes (Venezuela)

Published in SPIE Proceedings Vol. 6853:
Biomedical Optical Spectroscopy
Anita Mahadevan-Jansen; Robert R. Alfano; Alvin Katz; Wolfgang Petrich, Editor(s)

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