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The effect of motexafin gadolinium on ALA photodynamic therapy in glioma spheroids
Author(s): Marlon S. Mathews; Rogelio Sanchez; Chung-Ho Sun; Steen J. Madsen; Henry Hirschberg
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Paper Abstract

Following surgical removal of malignant brain tumors 80% of all cases develop tumor recurrence within 2 cm of the resected margin. The aim of postoperative therapy is therefore elimination of nests of tumor cells remaining in the margins of the resection cavity. Light attenuation in tissue makes it difficult for adequate light fluences to reach depths of 1-2 cm in the resection margin making it difficult for standard intraoperative photodynamic therapy (PDT) to accomplish this goal. Thus additional agents are required that either increase the efficacy of low fluence PDT or inhibit cellular repair, to enhance effectiveness of PDT in the tumor resection cavity. Motexafin gadolinium (MGd) is one such agent previously reported to enhance the cytotoxic potential of radiation therapy, as well as several chemotherapeutic agents by causing redox stress to cancerous cells. MGd is well tolerated with tumor specific uptake in clinical studies. The authors evaluated MGd as a potential PDT enhancing agent at low light fluences using an in vitro model. Multicellular Glioma spheroids (MGS) of approximately 300 micron diameter, obtained from ACBT cell lines were subjected to acute PDT treatments at 6J, 12J, and 18J light fluences. Growth was determined by measuring diameters in two axes. At four weeks a dose dependent inhibition of spheroid growth was seen in 33%, 55%, and 83% of the MGS at 6J, 12J, and 18J respectively, while inhibition followed by a partial reversal of growth was seen in 17%, 33%, and 17% respectively. This study provides a rationale for the use of this drug as a PDT enhancer in the management of brain tumors.

Paper Details

Date Published: 26 February 2008
PDF: 7 pages
Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 68422N (26 February 2008); doi: 10.1117/12.760260
Show Author Affiliations
Marlon S. Mathews, Beckman Laser Institute and Medical Clinic (United States)
Univ. of California, Irvine (United States)
Rogelio Sanchez, Beckman Laser Institute and Medical Clinic (United States)
Chung-Ho Sun, Beckman Laser Institute and Medical Clinic (United States)
Steen J. Madsen, Univ. of Nevada, Las Vegas (United States)
Henry Hirschberg, Beckman Laser Institute and Medical Clinic (United States)
Univ. of Nevada, Las Vegas (United States)

Published in SPIE Proceedings Vol. 6842:
Photonic Therapeutics and Diagnostics IV
Kenton W. Gregory; Guillermo J. Tearney; Reza S. Malek; Nikiforos Kollias; Bernard Choi; Haishan Zeng; Brian Jet-Fei Wong; Justus F. R. Ilgner; Henry Hirschberg; Steen J. Madsen, Editor(s)

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