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Proceedings Paper

Rapid and quantitative measuring of telomerase activity using an electro-chemiluminescent sensor
Author(s): Xiaoming Zhou; Da Xing; Debin Zhu; Li Jia
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Paper Abstract

Telomerase, a ribonucleoprotein enzyme that adds telomeric repeats to the 3'end of chromosomal DNA for maintaining chromosomal integrity and stability. This strong association of telomerase activity with tumors establishing it is the most widespread cancer marker. A number of assays based on the polymerase chain reaction (PCR) have been developed for the evaluation of telomerase activity. However, those methods require gel electrophoresis and some staining procedures. We developed an electrochemiluminescent (ECL) sensor for the measuring of telomerase activity to overcome these problems such as troublesome post-PCR procedures and semi-quantitative assessment in the conventional method. In this assay 5'-biotinylated telomerase synthesis (TS) primer serve as the substrate for the extension of telomeric repeats under telomerase. The extension products were amplified with this TS primer and a tris-(2'2'-bipyridyl) ruthenium (TBR)-labeled reversed primer. The amplified products was separated and enriched in the surface of electrode by streptavidin-coated magnetic beads, and detected by measuring the ECL signals of the TBR labeled. Measuring telomerase activity use the sensor is easy, sensitive, rapid, and applicable to quantitative analysis, should be clinically useful for the detection and monitoring of telomerase activity.

Paper Details

Date Published: 26 November 2007
PDF: 8 pages
Proc. SPIE 6830, Advanced Sensor Systems and Applications III, 68300L (26 November 2007); doi: 10.1117/12.760222
Show Author Affiliations
Xiaoming Zhou, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Debin Zhu, South China Normal Univ. (China)
Li Jia, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 6830:
Advanced Sensor Systems and Applications III
Yun-Jiang Rao; Yanbiao Liao; Gang-Ding Peng, Editor(s)

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